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Rapid chemical de-N-glycosylation and derivatization for liquid chromatography of immunoglobulin N-linked glycans
Glycan analysis may result in exploitation of glycan biomarkers and evaluation of heterogeneity of glycosylation of biopharmaceuticals. For N-linked glycan analysis, we investigated alkaline hydrolysis of the asparagine glycosyl carboxamide of glycoproteins as a deglycosylation reaction. By adding h...
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| Publié dans: | PLoS One |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Public Library of Science
2018
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5933716/ https://ncbi.nlm.nih.gov/pubmed/29723274 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0196800 |
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