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Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

When generating transgenic plants, generally the objective is to have stable expression of a transgene. This requires a single, intact integration of the transgene, as multi-copy integrations are often subjected to gene silencing. The Gateway-compatible binary vector based on bacterial artificial ch...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Tark-Dame, Mariliis, Weber, Blaise, de Sain, Mara, Anggoro, Damar Tri, Bader, Rechien, Walmsley, Aimee, Oka, Rurika, Stam, Maike
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5933267/
https://ncbi.nlm.nih.gov/pubmed/29658919
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57295
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