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Isolation of native plasma membrane H(+)‐ATPase (Pma1p) in both the active and basal activation states

The yeast plasma membrane H(+)‐ATPase Pma1p is a P‐type ATPase that energizes the yeast plasma membrane. Pma1p exists in two activation states: an autoinhibited basal state and an activated state. Here we show that functional and stable Pma1p can be purified in native form and reconstituted in artif...

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發表在:FEBS Open Bio
Main Authors: Pedersen, Jesper Torbøl, Kanashova, Tamara, Dittmar, Gunnar, Palmgren, Michael
格式: Artigo
語言:Inglês
出版: John Wiley and Sons Inc. 2018
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5929935/
https://ncbi.nlm.nih.gov/pubmed/29744292
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/2211-5463.12413
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