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Purification and Characterization of Caffeine Synthase from Tea Leaves

Caffeine synthase (CS), the S-adenosylmethionine-dependent N-methyltransferase involved in the last two steps of caffeine biosynthesis, was extracted from young tea (Camellia sinensis) leaves; the CS was purified 520-fold to apparent homogeneity and a final specific activity of 5.7 nkat mg(−1) prote...

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Detalhes bibliográficos
Main Authors: Kato, Misako, Mizuno, Kouichi, Fujimura, Tatsuhito, Iwama, Masanori, Irie, Masachika, Crozier, Alan, Ashihara, Hiroshi
Formato: Artigo
Idioma:Inglês
Publicado em: American Society of Plant Physiologists 1999
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC59297/
https://ncbi.nlm.nih.gov/pubmed/10364410
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