Long-term in vitro culture and preliminary establishment of chicken primordial germ cell lines

Primordial germ cells (PGCs) are precursors of functional gametes and can be used as efficient transgenic tools and carriers in bioreactors. Few methods for long-term culture of PGCs are available. In this study, we tested various culture conditions for PGCs, and used the optimum culture system to c...

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Bibliographic Details
Published in:PLoS One
Main Authors: Kong, Linglin, Qiu, Lingling, Guo, Qixin, Chen, Ying, Zhang, Xin, Chen, Bowen, Zhang, Yang, Chang, Guobin
Format: Artigo
Language:Inglês
Published: Public Library of Science 2018
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5927411/
https://ncbi.nlm.nih.gov/pubmed/29709001
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0196459
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