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Polymerase Chain Reaction with Confronting Two‐pair Primers for Polymorphism Genotyping

A novel PCR method using confronting two‐pair primers, named PCR‐CTPP, is introduced to detect a single nucleotide polymorphism (base X or Y). One primer for the X allele is set to include X'at the 3′end (antisense), where X'is the antisense of X, with the counterpart sense primer upstream...

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Publicat a:Jpn J Cancer Res
Autors principals: Hamajima, Nobuyuki, Saito, Toshiko, Matsuo, Keitaro, Kozaki, Ken‐ichi, Takahashi, Takashi, Tajima, Kazuo
Format: Artigo
Idioma:Inglês
Publicat: Blackwell Publishing Ltd 2000
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5926438/
https://ncbi.nlm.nih.gov/pubmed/11011111
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1349-7006.2000.tb01026.x
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