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Rapid CRISPR/Cas9-Mediated Cloning of Full-Length Epstein-Barr Virus Genomes from Latently Infected Cells

Herpesviruses have relatively large DNA genomes of more than 150 kb that are difficult to clone and sequence. Bacterial artificial chromosome (BAC) cloning of herpesvirus genomes is a powerful technique that greatly facilitates whole viral genome sequencing as well as functional characterization of...

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Detalhes bibliográficos
Publicado no:Viruses
Main Authors: Yajima, Misako, Ikuta, Kazufumi, Kanda, Teru
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5923465/
https://ncbi.nlm.nih.gov/pubmed/29614006
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/v10040171
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