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Application of droplet digital PCR in the analysis of genome integration and organization of the transgene in BAC transgenic mice

Transgenic (Tg) mice containing bacterial artificial chromosome (BAC) DNA are widely used for gene expression analysis and gene therapy models because BAC transgenes provide gene expression at physiological levels with the same developmental timing as endogenous genes. To ensure correct interpretati...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Nakagaki, Ayumi, Urakawa, Asuka, Hirano, Shiori, Anami, Takeru, Kishino, Tatsuya
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5923295/
https://ncbi.nlm.nih.gov/pubmed/29703985
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-25001-x
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