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Detection of Proteins that Recognize Platinum‐modified DNA Using Gel Mobility Shift Assay
Using a gel mobility shift assay, we have identified proteins, in the nuclear extracts of a human lung cancer cell line, that recognize cis‐diamminedichloroplatinum(II) (cis‐DDP, CDDP)‐modified DNA. A 158‐base‐pair double‐stranded DNA fragment, derived from pBR322 plasmid DNA, was modified by either...
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Publicado no: | Jpn J Cancer Res |
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Main Authors: | , , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Blackwell Publishing Ltd
1990
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5918002/ https://ncbi.nlm.nih.gov/pubmed/2125989 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1349-7006.1990.tb02680.x |
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