Construction of Mouse A9 Clones Containing a Single Human Chromosome Tagged with Neomycin‐resistance Gene via Microcell Fusion

Normal human fibroblasts (MRC‐5 or NTI‐4) were transfected with pSV2‐neo plasmid DNA. Fifty G418‐resistant fibroblast clones were isolated and independently fused to mouse A9 cells. The cell hybrids were selected and isolated in the medium containing G418 plus ouabaln. Since micronuclei were more ef...

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Detaylı Bibliyografya
Yayımlandı:Jpn J Cancer Res
Asıl Yazarlar: Koi, Minoru, Shimizu, Motoyuki, Morita, Hiroyuki, Yamada, Hideto, Oshimura, Mitsuo
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Blackwell Publishing Ltd 1989
Konular:
Rapid Communication
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC5917765/
https://ncbi.nlm.nih.gov/pubmed/2502516
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1349-7006.1989.tb02329.x
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