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A simple antegrade perfusion method for isolating viable single cardiomyocytes from neonatal to aged mice

The aim of this study was to establish a simple and reproducible antegrade perfusion method for isolating single viable mouse heart cells and to determine the standard practical protocols that are appropriate for mice of various ages. Antegrade perfusion was performed by injecting perfusate from nea...

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Detalles Bibliográficos
Publicado en:Physiol Rep
Main Authors: Omatsu‐Kanbe, Mariko, Yoshioka, Kengo, Fukunaga, Ryo, Sagawa, Hironori, Matsuura, Hiroshi
Formato: Artigo
Idioma:Inglês
Publicado: John Wiley and Sons Inc. 2018
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5917088/
https://ncbi.nlm.nih.gov/pubmed/29696821
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.14814/phy2.13688
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