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In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity

Small ubiquitin-like modifier (SUMO) modification is an important post-translational modification (PTM) that mediates signal transduction primarily through modulating protein-protein interactions. Similar to ubiquitin modification, SUMOylation is directed by a sequential enzyme cascade including E1-...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Yang, Wan-Shan, Campbell, Mel, Kung, Hsing-Jien, Chang, Pei-Ching
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5912215/
https://ncbi.nlm.nih.gov/pubmed/29443041
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56629
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