Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

One method extensively used for the quantification of gene expression changes and transcript abundances is reverse-transcription quantitative real-time PCR (RT-qPCR). It provides accurate, sensitive, reliable, and reproducible results. Several factors can affect the sensitivity and specificity of RT...

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Detalles Bibliográficos
Publicado en:J Vis Exp
Main Authors: Hashemipetroudi, Seyyed Hamidreza, Nematzadeh, Ghorbanali, Ahmadian, Gholamreza, Yamchi, Ahad, Kuhlmann, Markus
Formato: Artigo
Idioma:Inglês
Publicado: MyJove Corporation 2018
Assuntos:
Genetics
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5908672/
https://ncbi.nlm.nih.gov/pubmed/29443017
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55451
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