Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing

The complete genome of the channel catfish, Ictalurus punctatus, has been sequenced, leading to greater opportunities for studying channel catfish gene function. Gene knockout has been used to study these gene functions in vivo. The clustered regularly interspaced short palindromic repeats/CRISPR as...

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Foilsithe in:J Vis Exp
Main Authors: Elaswad, Ahmed, Khalil, Karim, Cline, David, Page-McCaw, Patrick, Chen, Wenbiao, Michel, Maximilian, Cone, Roger, Dunham, Rex
Formáid: Artigo
Teanga:Inglês
Foilsithe: MyJove Corporation 2018
Ábhair:
Genetics
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5908666/
https://ncbi.nlm.nih.gov/pubmed/29443028
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56275
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