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Phloem Sap Sampling from Brassica napus for 3D-PAGE of Protein and Ribonucleoprotein Complexes

Sampling the phloem of higher plants is often laborious and significantly dependent on the plant species. However, proteome studies under denaturing conditions could be achieved in different plant species. Native protein:protein and protein:nucleic acid complexes from phloem samples have as yet scar...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Pahlow, Steffen, Ostendorp, Anna, Krüßel, Lena, Kehr, Julia
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5908547/
https://ncbi.nlm.nih.gov/pubmed/29364282
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57097
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