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Culturing In Vivo-like Murine Astrocytes Using the Fast, Simple, and Inexpensive AWESAM Protocol

The AWESAM (a low-cost easy stellate astrocyte method) protocol entails a fast, simple, and inexpensive way to generate large quantities of in vivo-like mouse and rat astrocyte monocultures: Brain cells can be isolated from different brain regions, and after a week of cell culture, non-astrocytic ce...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:J Vis Exp
Main Authors: Wolfes, Anne C., Dean, Camin
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2018
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5908482/
https://ncbi.nlm.nih.gov/pubmed/29364267
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56092
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