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The role of temperature and bivalent ions in preparing competent Escherichia coli
Several factors including the culture temperature, bivalent ion, and osmotic stress were gradually optimized for preparing efficient Escherichia coli competent cells. The effect of culture temperature on the transformation efficiency (TrE) of E. coli DH5α was tested with 100 mM CaCl(2). The lower cu...
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| 出版年: | 3 Biotech |
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| 主要な著者: | , , , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Springer Berlin Heidelberg
2018
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5904042/ https://ncbi.nlm.nih.gov/pubmed/29682441 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-018-1243-x |
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