Introducing automation to the molecular diagnosis of Trypanosoma cruzi infection: A comparative study of sample treatments, DNA extraction methods and real-time PCR assays

BACKGROUND: Polymerase chain reaction (PCR) has become a useful tool for the diagnosis of Trypanosoma cruzi infection. The development of automated DNA extraction methodologies and PCR systems is an important step toward the standardization of protocols in routine diagnosis. To date, there are only...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Abras, Alba, Ballart, Cristina, Llovet, Teresa, Roig, Carme, Gutiérrez, Cristina, Tebar, Silvia, Berenguer, Pere, Pinazo, María-Jesús, Posada, Elizabeth, Gascón, Joaquim, Schijman, Alejandro G., Gállego, Montserrat, Muñoz, Carmen
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2018
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5903661/
https://ncbi.nlm.nih.gov/pubmed/29664973
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0195738
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