Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples

Genome sequencing has become a powerful tool for studying emerging infectious diseases; however, genome sequencing directly from clinical samples without isolation remains challenging for viruses such as Zika, where metagenomic sequencing methods may generate insufficient numbers of viral reads. Her...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Quick, Josh, Grubaugh, Nathan D, Pullan, Steven T, Claro, Ingra M, Smith, Andrew D, Gangavarapu, Karthik, Oliveira, Glenn, Robles-Sikisaka, Refugio, Rogers, Thomas F, Beutler, Nathan A, Burton, Dennis R, Lewis-Ximenez, Lia Laura, de Jesus, Jaqueline Goes, Giovanetti, Marta, Hill, Sarah, Black, Allison, Bedford, Trevor, Carroll, Miles W, Nunes, Marcio, Alcantara, Luiz Carlos, Sabino, Ester C, Baylis, Sally A, Faria, Nuno, Loose, Matthew, Simpson, Jared T, Pybus, Oliver G, Andersen, Kristian G, Loman, Nicholas J
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5902022/
https://ncbi.nlm.nih.gov/pubmed/28538739
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.066
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