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Central Metabolic Responses to the Overproduction of Fatty Acids in Escherichia coli Based on (13)C-Metabolic Flux Analysis
We engineered a fatty acid overproducing E. coli strain through overexpressing tesA (“pull”) and fadR (“push”) and knocking out fadE (“block”). This “pull-push-block” strategy yielded 0.17 gram of fatty acids (C(12)-C(18)) per gram of glucose (equivalent to 48% of the maximum theoretical yield) in b...
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| Publicado no: | Biotechnol Bioeng |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5901677/ https://ncbi.nlm.nih.gov/pubmed/24122357 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.25124 |
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