Purification and Characterization of a Novel Pumpkin Short-Chain Acyl-Coenzyme A Oxidase with Structural Similarity to Acyl-Coenzyme A Dehydrogenases

A novel pumpkin (Cucurbita pepo) short-chain acyl-coenzyme A (CoA) oxidase (ACOX) was purified to homogeneity by hydrophobic-interaction, hydroxyapatite, affinity, and anion-exchange chromatography. The purified enzyme is a tetrameric protein, consisting of apparently identical 47-kD subunits. The p...

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Udgivet i:	Plant Physiol
Principais autores:	De Bellis, Luigi, Gonzali, Silvia, Alpi, Amedeo, Hayashi, Hiroshi, Hayashi, Makoto, Nishimura, Mikio
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 2000
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC59006/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10806249/ https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.123.1.327
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