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Bacterial Cell Wall Precursor Phosphatase Assays Using Thin-layer Chromatography (TLC) and High Pressure Liquid Chromatography (HPLC)

Peptidoglycan encases the bacterial cytoplasmic membrane to protect the cell from lysis due to the turgor. The final steps of peptidoglycan synthesis require a membrane-anchored substrate called lipid II, in which the peptidoglycan subunit is linked to the carrier lipid undecaprenol via a pyrophosph...

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Bibliographic Details
Published in:Bio Protoc
Main Authors: Pazos, Manuel, Otten, Christian, Vollmer, Waldemar
Format: Artigo
Language:Inglês
Published: Bio-Protocol 2018
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5892711/
https://ncbi.nlm.nih.gov/pubmed/29651453
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2761
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