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Organotypic Explants of the Embryonic Rodent Hippocampus: An Accessible System for Transgenesis

This protocol describes the technique of ex-vivo electroporation to target embryonic hippocampal progenitors in an organotypic slice preparation. This technique allows gene perturbation for examining developmental processes in the embryonic hippocampus while retaining the environment and connectivit...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:Bio Protoc
Päätekijät: Iyer, Archana, Subramanian, Lakshmi, Tole, Shubha
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Bio-Protocol 2018
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC5884433/
https://ncbi.nlm.nih.gov/pubmed/29629394
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2764
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