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Centrifugation-free extraction of circulating nucleic acids using immiscible liquid under vacuum pressure

Extraction of cell-free DNA (cfDNA), which exists at an extremely low concentration in plasma, is a critical process for either targeted-sensing or massive sequencing of DNAs. However, such small amount of DNA cannot be fully obtained without high-speed centrifugation (<20,000 g). Here, we develo...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Lee, Hoyoon, Na, Wonhwi, Park, Chanhee, Park, Kyong Hwa, Shin, Sehyun
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5883035/
https://ncbi.nlm.nih.gov/pubmed/29615736
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-23766-9
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