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Enhanced labeling density and whole-cell 3D dSTORM imaging by repetitive labeling of target proteins

With continuing advances in the resolving power of super-resolution microscopy, the inefficient labeling of proteins with suitable fluorophores becomes a limiting factor. For example, the low labeling density achieved with antibodies or small molecule tags limits attempts to reveal local protein nan...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Sci Rep
Hauptverfasser: Venkataramani, Varun, Kardorff, Markus, Herrmannsdörfer, Frank, Wieneke, Ralph, Klein, Alina, Tampé, Robert, Heilemann, Mike, Kuner, Thomas
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5882651/
https://ncbi.nlm.nih.gov/pubmed/29615726
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-23818-0
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