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Isolation of intact RNA from murine CD4(+) T cells after intracellular cytokine staining and fluorescence-activated cell sorting
Intracellular cytokine staining (ICS) is a powerful method for identifying functionally distinct lymphocyte subsets, and for isolating these by fluorescence activated cell sorting (FACS). Although transcriptomic analysis of cells sorted on the basis of ICS has many potential applications, this is ra...
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| Опубликовано в: : | J Immunol Methods |
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| Главные авторы: | , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2018
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5878738/ https://ncbi.nlm.nih.gov/pubmed/29458078 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2018.02.008 |
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