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Isolation of intact RNA from murine CD4(+) T cells after intracellular cytokine staining and fluorescence-activated cell sorting

Intracellular cytokine staining (ICS) is a powerful method for identifying functionally distinct lymphocyte subsets, and for isolating these by fluorescence activated cell sorting (FACS). Although transcriptomic analysis of cells sorted on the basis of ICS has many potential applications, this is ra...

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Библиографические подробности
Опубликовано в: :J Immunol Methods
Главные авторы: Kunnath-Velayudhan, Shajo, Porcelli, Steven A.
Формат: Artigo
Язык:Inglês
Опубликовано: 2018
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5878738/
https://ncbi.nlm.nih.gov/pubmed/29458078
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2018.02.008
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