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Isolation of intact RNA from murine CD4(+) T cells after intracellular cytokine staining and fluorescence-activated cell sorting

Intracellular cytokine staining (ICS) is a powerful method for identifying functionally distinct lymphocyte subsets, and for isolating these by fluorescence activated cell sorting (FACS). Although transcriptomic analysis of cells sorted on the basis of ICS has many potential applications, this is ra...

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Bibliografische gegevens
Gepubliceerd in:J Immunol Methods
Hoofdauteurs: Kunnath-Velayudhan, Shajo, Porcelli, Steven A.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2018
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5878738/
https://ncbi.nlm.nih.gov/pubmed/29458078
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2018.02.008
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