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Single-cell qPCR facilitates the optimization of hematopoietic differentiation in hPSCs/OP9 coculture system

Human pluripotent stem cells (hPSCs)/OP9 coculture system is a widely used hematopoietic differentiation approach. The limited understanding of this process leads to its low efficiency. Thus, we used single-cell qPCR to reveal the gene expression profiles of individual CD34(+) cells from different s...

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Podrobná bibliografie
Vydáno v:Braz J Med Biol Res
Hlavní autoři: Chen, Haide, Jiang, Mengmeng, Xiao, Lei, Huang, He
Médium: Artigo
Jazyk:Inglês
Vydáno: Associação Brasileira de Divulgação Científica 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5875903/
https://ncbi.nlm.nih.gov/pubmed/29561959
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1590/1414-431X20187183
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