Evaluation of Two Protein Extraction Protocols Based on Freezing and Mechanical Disruption for Identifying Nontuberculous Mycobacteria by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry from Liquid and Solid Cultures

Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) has proved to be a useful diagnostic method for identifying conventional bacteria. In the case of mycobacteria, a good protein extraction protocol is essential in order to obtain reliable identification resul...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Main Authors: Rodriguez-Temporal, David, Perez-Risco, Daniel, Struzka, Eduardo A., Mas, Mireia, Alcaide, Fernando
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2018
Assuntos:
Mycobacteriology and Aerobic Actinomycetes
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5869846/
https://ncbi.nlm.nih.gov/pubmed/29367300
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01548-17
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