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Improved methods of DNA extraction from human spermatozoa that mitigate experimentally-induced oxidative DNA damage

Current approaches for DNA extraction and fragmentation from mammalian spermatozoa provide several challenges for the investigation of the oxidative stress burden carried in the genome of male gametes. Indeed, the potential introduction of oxidative DNA damage induced by reactive oxygen species, red...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Xavier, Miguel J., Nixon, Brett, Roman, Shaun D., Aitken, Robert John
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5868848/
https://ncbi.nlm.nih.gov/pubmed/29579126
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0195003
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