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An efficient method for integration of PCR fragments into adjacent or overlapping restriction sites during gene cloning
In the present work, a simple and straightforward method was developed to clone any PCR-amplified products into restriction sites that are very close, adjacent or overlapping in the expression vector. The novelty of the methodology involves a crucial primer-designing step by adding appropriate overh...
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| Publié dans: | 3 Biotech |
|---|---|
| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Springer Berlin Heidelberg
2018
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5866252/ https://ncbi.nlm.nih.gov/pubmed/29581929 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-018-1214-2 |
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