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A simple and robust protocol for high-yield expression of perdeuterated proteins in Escherichia coli grown in shaker flasks
We present a simple, convenient and robust protocol for expressing perdeuterated proteins in E coli BL21(DE3) cells in shaker flasks that reduces D(2)O usage ten-fold and d(7)-glucose usage by 30%. Using a modified M9 medium and optimized growth conditions, we were able to grow cells in linear log p...
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| Pubblicato in: | J Biomol NMR |
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| Autori principali: | , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2016
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5866134/ https://ncbi.nlm.nih.gov/pubmed/27709314 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-016-0052-y |
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