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Methodology challenges in studying human gut microbiota – effects of collection, storage, DNA extraction and next generation sequencing technologies

The information on microbiota composition in the human gastrointestinal tract predominantly originates from the analyses of human faeces by application of next generation sequencing (NGS). However, the detected composition of the faecal bacterial community can be affected by various factors includin...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Panek, Marina, Čipčić Paljetak, Hana, Barešić, Anja, Perić, Mihaela, Matijašić, Mario, Lojkić, Ivana, Vranešić Bender, Darija, Krznarić, Željko, Verbanac, Donatella
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5865204/
https://ncbi.nlm.nih.gov/pubmed/29572539
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-23296-4
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