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Expression, Purification, and Electrophysiological Characterization of a Recombinant, Fluorescent Kir6.2 in Mammalian Cells

The inwardly rectifying K(+) (Kir) channel, Kir6.2, plays critical roles in physiological processes in the brain, heart, and pancreas. Although Kir6.2 has been extensively studied in numerous expression systems, a comprehensive description of an expression and purification protocol has not been repo...

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Detalhes bibliográficos
Publicado no:Protein Expr Purif
Main Authors: Agasid, Mark T., Wang, Xuemin, Huang, Yiding, Janczak, Colleen M., Branström, Robert, Saavedra, S. Scott, Aspinwall, Craig A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5864551/
https://ncbi.nlm.nih.gov/pubmed/29409958
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2018.01.015
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