Endogenous peroxidases in sputum interfere with horse-radish peroxidase-based ELISAs

Peroxidase-based immunoassays are commonly used for detecting inflammatory mediators in biological samples. We suggest caution while interpreting assays particularly in sputum samples that have endogenous peroxidases like eosinophil peroxidase (EPX), which may interact with a horseradish peroxidase...

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Detalhes bibliográficos
Publicado no:J Immunol Methods
Main Authors: Kim, Lisa Ha-Yeon, Plaza, Karin, Thomas, Sruthi R., Draijer, Christina, Radford, Katherine, Peters-Golden, Marc, Mukherjee, Manali, Nair, Parameswaran
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5863226/
https://ncbi.nlm.nih.gov/pubmed/29154772
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2017.11.005
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