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Fluorescent Single-Stranded DNA-Binding Proteins Enable In Vitro and In Vivo Studies
Fluorescent single-stranded DNA-binding proteins (SSB) that have a defined number of fluorophores per tetramer are invaluable tools to understand biochemical mechanism and biological function. Here, we describe the purification of fluorescent SSB chimeras with a unique number of fluorescent subunits...
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| Publicado no: | Methods Mol Biol |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5862424/ https://ncbi.nlm.nih.gov/pubmed/22976191 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-032-8_18 |
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