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Monitoring ligand-dependent assembly of receptor ternary complexes in live cells by BRETFect

There is currently an unmet need for versatile techniques to monitor the assembly and dynamics of ternary complexes in live cells. Here we describe bioluminescence resonance energy transfer with fluorescence enhancement by combined transfer (BRETFect), a high-throughput technique that enables robust...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Cotnoir-White, David, El Ezzy, Mohamed, Boulay, Pierre-Luc, Rozendaal, Marieke, Bouvier, Michel, Gagnon, Etienne, Mader, Sylvie
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5856531/
https://ncbi.nlm.nih.gov/pubmed/29487210
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1716224115
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