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Chapter 17: Mechanical deformation of cultured cells with hydrogels

Polyacrylamide hydrogels can be used to culture cells in a range of stiffness that can closer mimic physiological environments. Changes in environmental stiffness have been documented in conditions such as fibrosis, cancer, and aging. In this chapter, we describe a method in which we pour gels direc...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Methods Mol Biol
Hauptverfasser: Worthen, Christal A., Rittié, Laure, Fisher, Gary J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2017
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5854178/
https://ncbi.nlm.nih.gov/pubmed/28836207
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7113-8_17
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