Influence of fecal collection conditions and 16S rRNA gene sequencing at two centers on human gut microbiota analysis

To optimise fecal sampling for reproducible analysis of the gut microbiome, we compared different methods of sample collection and sequencing of 16S rRNA genes at two centers. Samples collected from six individuals on three consecutive days were placed in commercial collection tubes (OMNIgeneGut OMR...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Penington, Jocelyn Sietsma, Penno, Megan A. S., Ngui, Katrina M., Ajami, Nadim J., Roth-Schulze, Alexandra J., Wilcox, Stephen A., Bandala-Sanchez, Esther, Wentworth, John M., Barry, Simon C., Brown, Cheryl Y., Couper, Jennifer J., Petrosino, Joseph F., Papenfuss, Anthony T., Harrison, Leonard C.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5847573/
https://ncbi.nlm.nih.gov/pubmed/29531234
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-22491-7
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