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An improved MS2 system for accurate reporting of the mRNA life cycle

The MS2-MCP system allows imaging multiple steps of the mRNA life cycle with high temporal and spatial resolution. However for short-lived mRNAs, the tight binding of the MS2 coat protein (MCP) to the MS2 binding sites (MBS) protects the RNA from being efficiently degraded, confounding the study of...

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Bibliografiske detaljer
Udgivet i:Nat Methods
Main Authors: Tutucci, Evelina, Vera, Maria, Biswas, Jeetayu, Garcia, Jennifer, Parker, Roy, Singer, Robert H.
Format: Artigo
Sprog:Inglês
Udgivet: 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5843578/
https://ncbi.nlm.nih.gov/pubmed/29131164
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4502
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