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The importance of post-thaw subculture for standardizing cellular activity of fresh or cryopreserved mouse embryonic stem cells

OBJECTIVE: Remarkable difference in cellular activity was found between early and late subpassaged embryonic stem cell (ESCs) lines, which can be created by subtle changes in cell manipulation protocol. This study subsequently examined whether post-thaw subculture of early subpassaged ESC lines coul...

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Detalhes bibliográficos
Publicado no:Asian-Australas J Anim Sci
Main Authors: Ko, Dong Woo, Yoon, Jung Ki, Ahn, Jong il, Lee, Myungook, Yang, Woo Sub, Ahn, Ji Yeon, Lim, Jeong Mook
Formato: Artigo
Idioma:Inglês
Publicado em: Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5838338/
https://ncbi.nlm.nih.gov/pubmed/28823125
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.5713/ajas.17.0294
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