A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging

Dense fluorophore labeling without compromising the biological target is crucial for genuine super-resolution microscopy. Here we introduce a broadly applicable labeling strategy for fixed and living cells utilizing a short peptide tag-specific nanobody (BC2-tag/bivBC2-Nb). BC2-tagging of ectopicall...

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Publicado en:Nat Commun
Main Authors: Virant, David, Traenkle, Bjoern, Maier, Julia, Kaiser, Philipp D., Bodenhöfer, Mona, Schmees, Christian, Vojnovic, Ilijana, Pisak-Lukáts, Borbála, Endesfelder, Ulrike, Rothbauer, Ulrich
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2018
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5834503/
https://ncbi.nlm.nih.gov/pubmed/29500346
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-018-03191-2
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