‘Cyclical Bias’ in Microbiome Research Revealed by A Portable Germ-Free Housing System Using Nested Isolation

Germ-Free (GF) research has required highly technical pressurized HEPA-ventilation anchored systems for decades. Herein, we validated a GF system that can be easily implemented and portable using Nested Isolation (NesTiso). GF-standards can be achieved housing mice in non-HEPA-static cages, which on...

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Vydáno v:Sci Rep
Hlavní autoři: Rodriguez-Palacios, Alexander, Aladyshkina, Natalia, Ezeji, Jessica C., Erkkila, Hailey L., Conger, Mathew, Ward, John, Webster, Joshua, Cominelli, Fabio
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2018
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5830500/
https://ncbi.nlm.nih.gov/pubmed/29491439
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-20742-1
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