Efficient Large-Scale 2D Culture System for Human Induced Pluripotent Stem Cells and Differentiated Cardiomyocytes

Cardiac regenerative therapies utilizing human induced pluripotent stem cells (hiPSCs) are hampered by ineffective large-scale culture. hiPSCs were cultured in multilayer culture plates (CPs) with active gas ventilation (AGV), resulting in stable proliferation and pluripotency. Seeding of 1 × 10(6)...

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Pubblicato in:Stem Cell Reports
Autori principali: Tohyama, Shugo, Fujita, Jun, Fujita, Chihana, Yamaguchi, Miho, Kanaami, Sayaka, Ohno, Rei, Sakamoto, Kazuho, Kodama, Masami, Kurokawa, Junko, Kanazawa, Hideaki, Seki, Tomohisa, Kishino, Yoshikazu, Okada, Marina, Nakajima, Kazuaki, Tanosaki, Sho, Someya, Shota, Hirano, Akinori, Kawaguchi, Shinji, Kobayashi, Eiji, Fukuda, Keiichi
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier 2017
Soggetti:
Report
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5829307/
https://ncbi.nlm.nih.gov/pubmed/28988990
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2017.08.025
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