Synchrotron Time-Lapse Imaging of Lignocellulosic Biomass Hydrolysis: Tracking Enzyme Localization by Protein Autofluorescence and Biochemical Modification of Cell Walls by Microfluidic Infrared Microspectroscopy

Tracking enzyme localization and following the local biochemical modification of the substrate should help explain the recalcitrance of lignocellulosic plant cell walls to enzymatic degradation. Time-lapse studies using conventional imaging require enzyme labeling and following the biochemical modif...

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Detalhes bibliográficos
Publicado no:Front Plant Sci
Main Authors: Devaux, Marie-Françoise, Jamme, Frédéric, André, William, Bouchet, Brigitte, Alvarado, Camille, Durand, Sylvie, Robert, Paul, Saulnier, Luc, Bonnin, Estelle, Guillon, Fabienne
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2018
Assuntos:
Plant Science
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5826215/
https://ncbi.nlm.nih.gov/pubmed/29515611
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fpls.2018.00200
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