A generic HTS assay for kinase screening: Validation for the isolation of an engineered malate kinase

An end-point ADP/NAD(+) acid/alkali assay procedure, directly applicable to library screening of any type of ATP-utilising/ADP producing enzyme activity, was implemented. Typically, ADP production is coupled to NAD(+) co-enzyme formation by the conventional addition of pyruvate kinase and lactate de...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Irague, Romain, Topham, Christopher M., Martineau, Nelly, Baylac, Audrey, Auriol, Clément, Walther, Thomas, François, Jean-Marie, André, Isabelle, Remaud-Siméon, Magali
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2018
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5819781/
https://ncbi.nlm.nih.gov/pubmed/29462203
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0193036
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