Small‐angle X‐ray and neutron scattering demonstrates that cell‐free expression produces properly formed disc‐shaped nanolipoprotein particles

Nanolipoprotein particles (NLPs), composed of membrane scaffold proteins and lipids, have been used to support membrane proteins in a native‐like bilayer environment for biochemical and structural studies. Traditionally, these NLPs have been prepared by the controlled removal of detergent from a det...

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Udgivet i:Protein Sci
Main Authors: Cleveland, Thomas E., He, Wei, Evans, Angela C., Fischer, Nicholas O., Lau, Edmond Y., Coleman, Matthew A., Butler, Paul
Format: Artigo
Sprog:Inglês
Udgivet: John Wiley and Sons Inc. 2018
Fag:
Methods and Applications
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5818747/
https://ncbi.nlm.nih.gov/pubmed/29266475
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3365
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