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Optimization of a Relative Telomere Length Assay by Monochromatic Multiplex Real-Time Quantitative PCR on the LightCycler 480: Sources of Variability and Quality Control Considerations

Telomere length (TL) measurement is central to many biomedical research, population, and epidemiology studies, with promising potential as a clinical tool. Various assays are used to determine TL, depending on the type and size of the sample. We describe the detailed optimization of a monochromatic...

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Pubblicato in:J Mol Diagn
Autori principali: Hsieh, Anthony Y.Y., Saberi, Sara, Ajaykumar, Abhinav, Hukezalie, Kyle, Gadawski, Izabella, Sattha, Beheroze, Côté, Hélène C.F.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Investigative Pathology 2016
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5818633/
https://ncbi.nlm.nih.gov/pubmed/26972047
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmoldx.2016.01.004
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