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Rapid Detection of Plasmodium knowlesi by Isothermal Recombinase Polymerase Amplification Assay

In this study, we developed a recombinase polymerase amplification (RPA) assay for specific diagnosis of Plasmodium knowlesi. Genomic DNA was extracted from whole blood samples using a commercial kit. With incubation at 37°C, the samples were successfully amplified within 20 minutes. The end product...

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Détails bibliographiques
Publié dans:Am J Trop Med Hyg
Auteurs principaux: Lai, Meng-Yee, Ooi, Choo-Huck, Lau, Yee-Ling
Format: Artigo
Langue:Inglês
Publié: The American Society of Tropical Medicine and Hygiene 2017
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5817784/
https://ncbi.nlm.nih.gov/pubmed/28820700
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4269/ajtmh.17-0427
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