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Directional freezing for the cryopreservation of adherent mammalian cells on a substrate
Successfully cryopreserving cells adhered to a substrate would facilitate the growth of a vital confluent cell culture after thawing while dramatically shortening the post-thaw culturing time. Herein we propose a controlled slow cooling method combining initial directional freezing followed by gradu...
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| Publicado en: | PLoS One |
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| Autores principales: | , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Public Library of Science
2018
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5813933/ https://ncbi.nlm.nih.gov/pubmed/29447224 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0192265 |
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