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Hydroxylamine chemical digestion for insoluble extracellular matrix characterization

The extracellular matrix (ECM) is readily enriched by decellularizing tissues with non-denaturing detergents to solubilize and deplete the vast majority of cellular components. This approach has been used extensively to generate ECM scaffolds for regenerative medicine technologies and in 3D cell cul...

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Détails bibliographiques
Publié dans:J Proteome Res
Auteurs principaux: Barrett, Alexander S., Wither, Matthew J., Hill, Ryan C., Dzieciatkowska, Monika, D’Alessandro, Angelo, Reisz, Julie A., Hansen, Kirk C.
Format: Artigo
Langue:Inglês
Publié: 2017
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5802359/
https://ncbi.nlm.nih.gov/pubmed/28971683
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jproteome.7b00527
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